Digital Quantification of Proteins and mRNA in Single Mammalian Cells
نویسندگان
چکیده
منابع مشابه
investigation of single-user and multi-user detection methods in mc-cdma systems and comparison of their performances
در این پایان نامه به بررسی روش های آشکارسازی در سیستم های mc-cdma می پردازیم. با توجه به ماهیت آشکارسازی در این سیستم ها، تکنیک های آشکارسازی را می توان به دو دسته ی اصلی تقسیم نمود: آشکارسازی سیگنال ارسالی یک کاربر مطلوب بدون در نظر گرفتن اطلاعاتی در مورد سایر کاربران تداخل کننده که از آن ها به عنوان آشکارساز های تک کاربره یاد می شود و همچنین آشکارسازی سیگنال ارسالی همه ی کاربران فعال موجود در...
Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
Cytoplasmic mRNA movements ultimately determine the spatial distribution of protein synthesis. Although some mRNAs are compartmentalized in cytoplasmic regions, most mRNAs, such as housekeeping mRNAs or the poly-adenylated mRNA population, are believed to be distributed throughout the cytoplasm. The general mechanism by which all mRNAs may move, and how this may be related to localization, is u...
متن کاملGenome-wide parallel quantification of mRNA and protein levels and turnover in mammalian cells
Correspondence: Jana Wolf, Max Delbrück Centrum for Molecular Medicine, Robert-Rössle-Str. 10, D13092 Berlin, Germany, Tel.: +49 30 9406 2641, Fax.: +49 30 9406 2394, email: [email protected] Wei Chen, Max Delbrück Centrum for Molecular Medicine, Robert-Rössle-Str. 10, D13092 Berlin, Germany, Tel.: +49 30 9406 2995, Fax.: +49 30 9406 3068, email: [email protected] Matthias Selbach, M...
متن کاملAutomating single subunit counting of membrane proteins in mammalian cells.
Elucidating subunit stoichiometry of neurotransmitter receptors is preferably carried out in a mammalian expression system where the rules of native protein assembly are strictly obeyed. Although successful in Xenopus oocytes, single subunit counting, manually counting photobleaching steps of GFP-tagged subunits, has been hindered in mammalian cells by high background fluorescence, poor control...
متن کاملStochastic mRNA Synthesis in Mammalian Cells
Individual cells in genetically homogeneous populations have been found to express different numbers of molecules of specific proteins. We investigated the origins of these variations in mammalian cells by counting individual molecules of mRNA produced from a reporter gene that was stably integrated into the cell's genome. We found that there are massive variations in the number of mRNA molecul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Cell
سال: 2016
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2016.02.030